cd138 pe antibody (Miltenyi Biotec)
93
Structured Review
Miltenyi Biotec
cd138 pe antibody
Cd138 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd138+pe/CD138+Antibody%2C+anti-human%2C+REAfinity/pmc13373028-168-56-59
Average 93 stars, based on 10 article reviews
Cd138 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd138+pe/CD138+Antibody%2C+anti-human%2C+REAfinity/pmc13373028-168-56-59
Average 93 stars, based on 10 article reviews
cd138 pe antibody - by Bioz Stars,
2026-09
93/100 stars
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Incubation:Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow Article Snippet: Plasma cells were enriched from bone marrow using StraightFrom Whole Blood and Bone Marrow CD138 MicroBeads and StraightFrom Whole Blood CD19 MicroBeads (Miltenyi Biotec) according to manufacturer’s instructions. .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-VioBlue (BW264/56, Miltenyi Biotec, Cat. 130-113-133, 1:400), CD10-VioBlue (97C5, Miltenyi Biotec, Cat. 130-099-670, 1:11); CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200); CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: .. Caspase-3 active staining, mitochondrial membrane potential for the monitoring of the TMRM signal For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression Article Snippet: .. For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Blocking Assay:Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow Article Snippet: Plasma cells were enriched from bone marrow using StraightFrom Whole Blood and Bone Marrow CD138 MicroBeads and StraightFrom Whole Blood CD19 MicroBeads (Miltenyi Biotec) according to manufacturer’s instructions. .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-VioBlue (BW264/56, Miltenyi Biotec, Cat. 130-113-133, 1:400), CD10-VioBlue (97C5, Miltenyi Biotec, Cat. 130-099-670, 1:11); CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200); CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25) and Article Title: Disrupted callosal connectivity underlies long-lasting sensory-motor deficits in an NMDA receptor antibody encephalitis mouse model Article Snippet: .. Cell samples were blocked with FcR Block (Miltenyi Biotec) and stained with fluorescent antibodies to cell surface markers: IgD Brilliant Violet 421 (Biolegend 11-26c.2a), CD20 FITC (Beckman Coulter B9E9), CD38 PerCPCy5.5 (BioLegend HIT2), CD3 PE-Cy7 (Beckman UCHT1), Staining:Article Title: Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow Article Snippet: Plasma cells were enriched from bone marrow using StraightFrom Whole Blood and Bone Marrow CD138 MicroBeads and StraightFrom Whole Blood CD19 MicroBeads (Miltenyi Biotec) according to manufacturer’s instructions. .. Enriched cells were incubated with Fc Blocking Reagent (Miltenyi Biotec) following manufacturer’s instructions and subsequently stained for 30 min at 4 °C with the following anti-human antibodies: CD3-VioBlue (BW264/56, Miltenyi Biotec, Cat. 130-113-133, 1:400), CD10-VioBlue (97C5, Miltenyi Biotec, Cat. 130-099-670, 1:11); CD14-VioBlue (TÜK4, Miltenyi Biotec, Cat. 130-113-152, 1:200); CD38-APC (HIT2, BioLegend, Cat. 303510, 1:25) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: .. Caspase-3 active staining, mitochondrial membrane potential for the monitoring of the TMRM signal For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: Results were normalized to samples without treatment (100%) and analyzed using GraphPad Prism software. .. At the appropriate time point, activated B cells were washed, stained with either CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) for phase 2, either with CD20-FITC (Miltenyi Biotech; 130-113-429), CD38-APC (Miltenyi Biotech; 130-113-429) and Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine. Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8FITC (1:50, 130-120-822), Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8-FITC (1:50, 130-120-822), Article Title: Disrupted callosal connectivity underlies long-lasting sensory-motor deficits in an NMDA receptor antibody encephalitis mouse model Article Snippet: .. Cell samples were blocked with FcR Block (Miltenyi Biotec) and stained with fluorescent antibodies to cell surface markers: IgD Brilliant Violet 421 (Biolegend 11-26c.2a), CD20 FITC (Beckman Coulter B9E9), CD38 PerCPCy5.5 (BioLegend HIT2), CD3 PE-Cy7 (Beckman UCHT1), Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression Article Snippet: Results were normalized to samples without treatment (100%) and analyzed using GraphPad Prism software. .. At the appropriate time point, activated B cells were washed, stained with either CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) for phase 2, either with CD20-FITC (Miltenyi Biotech; 130-113-429), CD38-APC (Miltenyi Biotech; 130-113-429) and Membrane:Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: .. Caspase-3 active staining, mitochondrial membrane potential for the monitoring of the TMRM signal For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and In Vitro:Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: .. Caspase-3 active staining, mitochondrial membrane potential for the monitoring of the TMRM signal For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression Article Snippet: .. For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Cell Differentiation:Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: .. Caspase-3 active staining, mitochondrial membrane potential for the monitoring of the TMRM signal For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression Article Snippet: .. For the in vitro total B cell differentiation model, cells were incubated with CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) antibodies for phase 2, either with CD38-APC (BD Biosciences; 555462) and FACS:Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression. Article Snippet: Results were normalized to samples without treatment (100%) and analyzed using GraphPad Prism software. .. At the appropriate time point, activated B cells were washed, stained with either CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) for phase 2, either with CD20-FITC (Miltenyi Biotech; 130-113-429), CD38-APC (Miltenyi Biotech; 130-113-429) and Article Title: PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression Article Snippet: Results were normalized to samples without treatment (100%) and analyzed using GraphPad Prism software. .. At the appropriate time point, activated B cells were washed, stained with either CD38-APC (BD Biosciences; 555462) and CD23-PC.7 (BD Biosciences; 561167) for phase 2, either with CD20-FITC (Miltenyi Biotech; 130-113-429), CD38-APC (Miltenyi Biotech; 130-113-429) and Marker:Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine. Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8FITC (1:50, 130-120-822), Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8-FITC (1:50, 130-120-822), Expressing:Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine. Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8FITC (1:50, 130-120-822), Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8-FITC (1:50, 130-120-822), Fluorescence:Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine. Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8FITC (1:50, 130-120-822), Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine Article Snippet: For ex vivo analysis, mouse splenocytes were stimulated with 1 μg/mL of S peptide (SARS-CoV-2 S1 RBD recombinant protein, RP-87706, ThermoFisher Scientific, Waltham, MA, USA). .. For marker expression, cells were harvested after 36 h and stained with Viobility Fixable Dye (Miltenyi, Bologna, Italy) and CD3ε-APC (1:50, 130-117-671), CD4-APC-Vio770 (1:50, 130-118-955), CD8-FITC (1:50, 130-122-720), CD69-PE (1:50, 30-115-460), CD3-FITC (1:50, 130-092-962), CD4-VioBlue (1:50, 130-123-208), CD25-PE (1:50, 130-120-696), CD8-FITC (1:50, 130-120-822), |